The flow cytometer Diaries
The flow cytometer Diaries
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Facts-enriched characterization of cell populations. Morphological details from images can incorporate into the richness of flow cytometry knowledge. One example is, the determine exhibits an if not traditional apoptosis assay working with Annexin V and PI, incorporating cell imaging to characterize cells in Each individual population to expose morphologically distinctive options. These insights could not have been attained from multiplex staining on your own.
As samples are obtained over the Attune CytPix Flow Cytometer, the significant-pace brightfield digital camera captures and stores pictures of detected gatherings in a amount up to 6,000 photographs for every 2nd, dependant upon the flow level and graphic dimension. For bigger versatility, Attune Cytometric Computer software lets you alter the impression capture frequency as needed and select a specific gate for picture seize, if required.
Blend acoustic focusing technologies with large-parameter Assessment for flexible spectral unmixing and traditional workflows.
Discover new insights into your experimental facts by visualizing populations using mixed fluorescence and morphology information. Our software package interface wizards permit downsampling, concatenation (merged information) and dimensionality resources which might be created into the software program for an intuitive, streamlined details analysis practical experience.
"A picture is not quite well worth 1000 dots, but it definitely helps to see photos. It is great to find out photographs that correlate with staining. One example is, AnnexinV-optimistic cells that are morphologically diverse than wholesome cells, or CD14+ monocytes which have been more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it truly is illuminating to discover how many doublets slip as a result of conventional singlet gates, and great to verify the standing of unanticipated double-positive gatherings in my Examination."
Pair your Attune flow cytometer by having an autosampler for improved workflow performance, and include robotic integration for max throughput.
Constant graphic high quality even at higher throughput Acoustic focusing in addition to a significant-velocity camera Blend to impression these T cells constantly at reduced or superior flow prices. Conveniently regulate concentrate and digital camera settings to fulfill experimental prerequisites.
Early signs of this contamination had to begin with been dismissed as particles. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also enables you to use morphological features to find exciting subpopulations that will not be obvious from flow cytometry data by itself.
Buyers can perspective occasion illustrations or photos in true-time to confirm sample top quality, enhance protocols, and record the morphology of each mobile inhabitants discovered for Investigation. Imaging capture also benefits from acoustic concentrating, which helps you to situation the cells making sure that a sharp, centered graphic is acquired.
Seem waves allow exact cell alignment, resulting in Outstanding info high-quality and ~10X more rapidly sample flow rates.
Software Take note: Automatic image analysis cuts down person-to-consumer variability in flow cytometry gating methods
Leverage the strength of Innovative technologies and innovation to speed up your discoveries. Have confidence in inside the Attune Flow Cytometer spouse and children to deliver the trustworthiness, speed, and accuracy that established your perform apart.
Versatile payment plan from Agilent Financial Solutions permit you to obtain the vital instrumentation you need for quick task, or to improve your lab.
Gating on these extended parameters permits you to rapidly and properly detect populations of interest and make sure gating system with little if any guide review. The Attune program delivers usage of condition-of-the-artwork machine learning analysis applications in an expertly developed person interface, which makes it exceptionally easy to use.
As the mobile line had Earlier been analyzed about the Attune CytPix Flow Cytometer, the researchers went back to the photographs and were capable to doc the microbial an infection not less than 5 days before.
Agilent Value Assure read more guarantees you a minimum of 10 years of instrument use from the date you purchase. In any other case, We are going to credit you While using the residual price of the process towards an upgraded design.
What appeared to be a homogeneous inhabitants is in fact additional diverse—an insight that ought to be thought of when interpreting outcomes.